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Serum collection method and precautions

Serum:
Sample size requirements:
GC-MS, LC-MS experiments 200 µL/sample per platform.
NMR experiments 500 µL/sample.
If you do multiple platforms, in order to avoid repeated freezing and thawing, be sure to send them in separate packages.

Sample collection steps:
The blood was collected and placed in a centrifuge tube at 37 °C (or room temperature) for 1 h for coagulation and stratification. Then centrifuge at 3000 rpm for 10 min at room temperature, and transfer the supernatant to a clean centrifuge tube. Centrifuge at 12,000 rpm and 4 °C for 10 min, take the supernatant and aliquot into 1.5 mL centrifuge tubes, 0.2 mL per tube, and freeze at -80 °C. Sufficient dry ice delivery.

Precautions:
1) If alcohol is used for disinfection when taking blood, please dry the wiped area and take samples after the alcohol has completely evaporated.
2) If an anesthetic is used when taking blood, it is recommended to use isoflurane to prevent interference peaks in the NMR serum spectrum.
3) Serum reference yield: 30% to 50% (for example, 1 mL of whole blood can yield approximately 0.3 to 0.5 mL of serum).
4) If you use a vacuum blood collection tube when collecting whole blood, be sure to use a coagulation tube without pre-anticoagulant (the cap is red).
5) It is strongly recommended to collect as many samples as possible and aliquot them for cryopreservation.
P.S.:
1. It is recommended to use 1.5 mL centrifuge tubes for aliquot storage (this can be used for many experiments and avoid repeated freezing and thawing). The screw-top cryovials are not used because there is a rubber gasket in the cap of the screw-top cryovials. However, under the low temperature of -80 ℃ dry ice for a long time, the rubber ring will become hard and brittle, easy to fall off, resulting in poor sealing and sample leakage.
2. If some laboratories do not have a 12000 rpm centrifuge, this step can be skipped. Send the samples to note on the information sheet. After thawing, the experimenter can centrifuge for subsequent experiments.