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The correct way of using the Volumetric Pipette

Abstract: Volumetric Pipette is a gauge used to accurately move a certain volume of solution. A Volumetric Pipette is a dispensing instrument used only to measure the volume of the solution it releases. It is a long, thin glass tube with an expanded part in the middle. The lower end of the nozzle, the upper end of the tube neck is engraved with a line, is the accurate volume of the mark.

1 Introduction

Common Volumetric Pipettes have 5,10,25,50 and other specifications. The volume removed by the Volumetric Pipette is usually accurate to 0.01mL.

2. Steps

According to the volume and requirements of the solution to be moved, choose the appropriate size of the Volumetric Pipette for use. The Volumetric Pipette is generally used for accurate removal of the solution in titration analysis, and it is generally used when the reaction needs to control the amount of the test solution.

Inspection equipment

Check whether the nozzle and tip of the Volumetric Pipette are damaged or not. If there is any damage, it cannot be used.

Clean the instrument

After rinsing with tap water, soak with chromic acid detergent.

The operation method is as follows:

Take the upper end of the Volumetric Pipette in a suitable position with the right hand, index finger close to the upper mouth of the Volumetric Pipette, middle finger and ring finger open and hold the outside of the Volumetric Pipette, thumb in the middle of the middle finger and ring finger hold the inside of the Volumetric Pipette, little finger naturally relax;

Hold the ear washing ball in the left hand and hold the clenched fist position. Hold the ear suction ball in the palm with the tip down, clench the ear suction ball and discharge the air in the ball. Insert the tip of the ear suction ball into or close to the top of the Volumetric Pipette, pay attention to avoid air leakage.

Slowly loosen the left hand finger, the washing liquid slowly inhaled into the tube, until the scale above the part, remove the suction ear ball, quickly block the pipet mouth with the right index finger, wait for a moment, the washing liquid back to the original bottle. Rinse the inner and outer walls of the Volumetric Pipette with running water until water drops are not hanging, then wash with distilled water for 3 times, control the dry water for reserve.

Draw solution

Shake to solution absorption, will stay pour a small part of the solution absorption in a small beaker, wash and dry with a Volumetric Pipette tip of filter will be cleaned inside and outside the moisture blot, extract solution and insert small beaker and when suction to 1/3 of the pipet capacity, with right index finger hold the nozzle immediately, remove and a horizontal and turn the Volumetric Pipette, make the solution flows through the pipe wall, Dump the solution from the lower tip into the waste liquid cup. In this way, the solution can be absorbed after 3-4 times of moistening and washing.

Insert the Volumetric Pipette that has been moisten and washed with the liquid to be sucked into a place 1 ~ 2cm below the liquid to be sucked, and use the suction ear ball to absorb the solution as described above (note that the Volumetric Pipette should not be inserted too deep into the solution, and should be inserted downward while sucking, and always maintain this depth). When the tube head to located about 1 ~ 2 cm above the line, quickly with the right hand index finger plug nozzle (fall at this time if the solution to the tong, should learn from the back), the pipet to suction surface is put forward, and make the contact tube tip filed after a short stay liquid container wall, using filter paper dry the pipet lower adhesion of a small amount of solution. (When moving the Volumetric Pipette, the Volumetric Pipette should be kept vertical, not tilted.)

Regulating liquid level

Take another clean beaker in the left hand and place the tip of the Volumetric Pipette close to the inner wall of the beaker. Keep the beaker tilted so that the Volumetric Pipette is vertical and the scale line and the eye level (the left hand should not touch the Volumetric Pipette). Loosen the index finger slightly (you can turn the pipet slightly), so that the solution in the tube slowly flows out from the lower mouth. When the liquid level reaches the scale line, press the right index finger tightly, pause for a moment, and then press the upper method to put the bottom line of the meniscus of the solution to the tangent with the upper edge of the mark line, and immediately press the tube mouth with the index finger. Place the tip close to the inside of the beaker and move a little toward the beaker to remove any drops from the tip. Carefully move the Volumetric Pipette into the container receiving the solution.

Effluent solution

The pipet upright, receiver tilt, pipe bottom close to receiver walls, open your second finger, the solution along the receiver down walls, tube after the solution flow, keep stay 15 s fluid status, the Volumetric Pipette tip in receiver by point by wall slip a few small distance before and after (or rotate the Volumetric Pipette tip by receiver inside a week), Remove Volumetric Pipette (the small amount of solution remaining on the inner wall of the Volumetric Pipette tip should not be forced out by external force because the volume of the retained solution at the inner wall of the Volumetric Pipette tip has been taken into account when calibrating the Volumetric Pipette. )

Clean the instrument

Clean Volumetric Pipette and place on Volumetric Pipette rack.

3 Matters needing attention

1. Volumetric Pipette should not be dried in an oven.

2. The Volumetric Pipette should not move too hot or too cold a solution.

3. As far as possible, the same Volumetric Pipette should be used in the same experiment.

4. After the Volumetric Pipette is used, it should be immediately rinsed with tap water and distilled water and placed on the Volumetric Pipette rack.

5. Volumetric Pipette and volumetric flask are often used together, so the relative volume of the two is often calibrated before use.

6. In use, in order to reduce measurement error, each time from the top scale (0 scale) as the starting point, to lower the required volume of the solution, rather than absorb as much volume as needed.

As a   Volumetric Pipette manufacturer & supplier of medical equipment and equipment, Huida can not only provide customers with overall solutions for laboratory equipment, but also provide a full range of high-quality products and related services for laboratory glasswares. Welcome to send an inquiry for more details.